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University of Queensland Diamantina Institute

When scientists need to accurately and precisely deliver smaller volumes of a liquid, they use a pipette a calibrated glass tube into which the liquid is drawn and then released. Glass and plastic pipettes have been mainstays of chemistry and biology laboratories for decades, and they can be relied upon to dispense volumes down to 0.1mL.

Molecular biologists frequently use much smaller volumes of liquids cartier gold bangle fake in their work, even getting down to 0.1 (that one ten thousandth of a millilitre, or one ten millionth of a litre!). For such small volumes, they need to use a micropipette.

Micropipettes are called a lot of different names, most of which are based on the companies which manufacture. For example, you might hear them called as a large number of these devices used in laboratories are made by this company. Regardless of the manufacturer, micropipettes operate on the same principle: a plunger is depressed by the thumb and as it is released, liquid is drawn into a disposable plastic tip. When the plunger is pressed again, the liquid is dispensed.

The tips are an important part of the micropipette and allow the same device to be used for different samples (so long as you change your tip between samples) without washing. They come in a number of different sizes and replica 18k cartier love bracelet colours, depending on the micropipette they are used with, and the volume to be dispensed.

The most commonly used tips are:The plunger can sit at any one of three positions:

Keeping the plunger at the second position, place the tip attached to the end of the micropipette beneath the surface of the liquid to be drawn up. Try not to push right to the bottom (especially if you are removing supernatant from a centrifuged pellet), but ensure that the tip is far enough below the surface of the liquid fake gold love cartier bracelet that no air is drawn up.

Steadily release pressure on the plunger and allow it to return to Position 1. Do this carefully, particularly with large volumes, as the liquid may shoot up into the tip and the body of the micropipette. If bubbles cartier love bracelet solid gold copy appear in the tip, return the liquid to the container by pushing down to Position 3 and start again (you may need to change to a dry tip).

Changing the Volume

Some micropipettes deliver fixed volumes, however the majority are adjustable. Each brand uses a slightly different method to do this Gilsons have an adjustable wheel, others have a locking mechanism and turning the plunger adjusts the volume. All have a readout which tells you how much is being delivered and a range of volumes which can be dispensed. Trying to dipense less than the lower value of the range will result in inaccurate measurements. Trying to dispense over the upper range will completely fill the tip and allow liquid to enter the body of the pipette. Do not overwind the volume adjustment, as this affects the calibration of the micropipette.
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